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Examinando por Autor "Rojas-Villacorta, Walter"

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    Green Energy Generated in Single-Chamber Microbial Fuel Cells Using Tomato Waste
    (MDPI, 2023-07-03) Rojas-Flores, Segundo; De La Cruz-Noriega, Magaly; Cabanillas-Chirino, Luis; Benites, Santiago M.; Nazario-Naveda, Renny; Delfín-Narciso, Daniel; Gallozzo-Cardena, Moisés; Murga-Torres, Emzon; Rojas-Villacorta, Walter; Diaz, Félix
    This research used tomato waste as a substrate (fuel) in Single Chamber-Microbial Fuel Cells (scMFC) on a small scale. The electrochemical properties were monitored, the functional groups of the substrate were analyzed by Fourier Transform Infrared Spectrophotometry (FTIR) and a microbiological analysis was performed on the electrodes in order to identify the microorganisms responsible for the electrochemical process. The results show voltage peaks and an electrical current of 3.647 ± 0.157 mA and 0.957 ± 0.246 V. A pH of 5.32 ± 0.26 was measured in the substrate with an electrical current conductivity of 148,701 ± 5849 mS/cm and an internal resistance (Rint) of 77. 517 ± 8.541 Ω. The maximum power density (PD) displayed was 264.72 ± 3.54 mW/cm2 at a current density (CD) of 4.388 A/cm2. On the other hand, the FTIR spectrum showed a more intense decrease in its peaks, with the compound belonging to the phenolic groups being the most affected at 3361 cm−1. The micrographs show the formation of a porous biofilm where molecular identification allowed the identification of two bacteria (Proteus vulgaris and Proteus vulgaris) and a yeast (Yarrowia lipolytica) with 100% identity. The data found show the potential of this waste as a source of fuel for the generation of an electric current in a sustainable and environmentally friendly way, generating in the near future a mechanism for the reuse of waste in a beneficial way for farmers, communities and agro-industrial companies.
  • Cargando...
    Miniatura
    PublicaciónAcceso abierto
    Green Energy Generated in Single-Chamber Microbial Fuel Cells Using Tomato Waste
    (Multidisciplinary Digital Publishing Institute (MDPI), 2023-07-03) Rojas-Flores, Segundo; De La Cruz-Noriega, Magaly; Cabanillas-Chirinos, Luis; Benites, Santiago M.; Nazario-Naveda, Renny; Delfín-Narciso, Daniel; Gallozzo-Cardenas, Moisés; Diaz, Félix; Murga-Torres, Emzon; Rojas-Villacorta, Walter
    “This research used tomato waste as a substrate (fuel) in Single Chamber-Microbial Fuel Cells (scMFC) on a small scale. The electrochemical properties were monitored, the functional groups of the substrate were analyzed by Fourier Transform Infrared Spectrophotometry (FTIR) and a microbiological analysis was performed on the electrodes in order to identify the microorganisms responsible for the electrochemical process. The results show voltage peaks and an electrical current of 3.647 ± 0.157 mA and 0.957 ± 0.246 V. A pH of 5.32 ± 0.26 was measured in the substrate with an electrical current conductivity of 148,701 ± 5849 mS/cm and an internal resistance (Rint) of 77. 517 ± 8.541 Ω. The maximum power density (PD) displayed was 264.72 ± 3.54 mW/cm2 at a current density (CD) of 4.388 A/cm2 . On the other hand, the FTIR spectrum showed a more intense decrease in its peaks, with the compound belonging to the phenolic groups being the most affected at 3361 cm−1 . The micrographs show the formation of a porous biofilm where molecular identification allowed the identification of two bacteria (Proteus vulgaris and Proteus vulgaris) and a yeast (Yarrowia lipolytica) with 100% identity. The data found show the potential of this waste as a source of fuel for the generation of an electric current in a sustainable and environmentally friendly way, generating in the near future a mechanism for the reuse of waste in a beneficial way for farmers, communities and agro-industrial companies.“
  • Cargando...
    Miniatura
    PublicaciónAcceso abierto
    Impact of Dragon Fruit Waste in Microbial Fuel Cells to Generate Friendly Electric Energy
    (MDPI, 2023-03-27) Rojas-Flores, Segundo; Santiago, M. Benites; De La Cruz-Noriega, Magaly; Vives-Garnique, Juan; Milly Otiniano, Nélida; Rojas-Villacorta, Walter; Gallozzo-Cardenas, Moisés; Delfín-Narciso, Daniel; Díaz, Félix
    Pollution generated by the misuse of large amounts of fruit and vegetable waste has become a major environmental and social problem for developing countries due to the absence of specialized collection centers for this type of waste. This research aims to generate electricity in an eco-friendly way using red dragon fruit (pitahaya) waste as the fuel in single-chamber microbial fuel cells on a laboratory scale using zinc and copper electrodes. It was possible to generate voltage and current peaks of 0.46 ± 0.03 V and 2.86 ± 0.07 mA, respectively, with an optimum operating pH of 4.22 ± 0.09 and an electrical conductivity of 175.86 ± 4.72 mS/cm at 8 °Brix until the tenth day of monitoring. An internal resistance of 75.58 ± 5.89 Ω was also calculated with a maximum power density of 304.33 ± 16.51 mW/cm2 at a current density of 5.06 A/cm2, while the FTIR spectra showed a decrease in the initial compounds and endings, especially at the 3331 cm−1 peaks of the O–H bonds. Finally, the yeast-like fungus Geotrichum candidum was molecularly identified (99.59%). This research will provide great opportunities for the generation of renewable energy using biomass as fuel through electronic devices with great potential to generate electricity.
  • Cargando...
    Miniatura
    PublicaciónAcceso abierto
    “Impact of Dragon Fruit Waste in Microbial Fuel Cells to Generate Friendly Electric Energy“
    (MDPI, 2023-04-27) Rojas-Flores, Segundo; Santiago M., Benites; De La Cruz-Noriega, Magaly; Vives-Garnique, Juan; Milly Otiniano, Nélida; Rojas-Villacorta, Walter; Gallozzo-Cardenas, Moisés; Delfín-Narciso, Daniel; Díaz, Félix
    “Abstract: Pollution generated by the misuse of large amounts of fruit and vegetable waste has become a major environmental and social problem for developing countries due to the absence of specialized collection centers for this type of waste. This research aims to generate electricity in an eco-friendly way using red dragon fruit (pitahaya) waste as the fuel in single-chamber microbial fuel cells on a laboratory scale using zinc and copper electrodes. It was possible to generate voltage and current peaks of 0.46 ± 0.03 V and 2.86 ± 0.07 mA, respectively, with an optimum operating pH of 4.22 ± 0.09 and an electrical conductivity of 175.86 ± 4.72 mS/cm at 8 ◦Brix until the tenth day of monitoring. An internal resistance of 75.58 ± 5.89 Ω was also calculated with a maximum power density of 304.33 ± 16.51 mW/cm2 at a current density of 5.06 A/cm2 , while the FTIR spectra showed a decrease in the initial compounds and endings, especially at the 3331 cm−1 peaks of the O–H bonds. Finally, the yeast-like fungus Geotrichum candidum was molecularly identified (99.59%). This research will provide great opportunities for the generation of renewable energy using biomass as fuel through electronic devices with great potential to generate electricity.“
  • Cargando...
    Miniatura
    PublicaciónAcceso abierto
    “Impact of Dragon Fruit Waste in Microbial Fuel Cells to Generate Friendly Electric Energy“
    (MDPI, 2023-04-27) Rojas-Flores, Segundo; Benites, Santiago M.; De La Cruz-Noriega, Magaly; Vives-Garnique, Juan; Otiniano, Nélida Milly; Rojas-Villacorta, Walter; Gallozzo-Cardenas, Moisés; Delfín-Narciso, Daniel; Díaz, Félix
    “Abstract: Pollution generated by the misuse of large amounts of fruit and vegetable waste has become a major environmental and social problem for developing countries due to the absence of specialized collection centers for this type of waste. This research aims to generate electricity in an eco-friendly way using red dragon fruit (pitahaya) waste as the fuel in single-chamber microbial fuel cells on a laboratory scale using zinc and copper electrodes. It was possible to generate voltage and current peaks of 0.46 ± 0.03 V and 2.86 ± 0.07 mA, respectively, with an optimum operating pH of 4.22 ± 0.09 and an electrical conductivity of 175.86 ± 4.72 mS/cm at 8 ◦Brix until the tenth day of monitoring. An internal resistance of 75.58 ± 5.89 Ω was also calculated with a maximum power density of 304.33 ± 16.51 mW/cm2 at a current density of 5.06 A/cm2 , while the FTIR spectra showed a decrease in the initial compounds and endings, especially at the 3331 cm−1 peaks of the O–H bonds. Finally, the yeast-like fungus Geotrichum candidum was molecularly identified (99.59%). This research will provide great opportunities for the generation of renewable energy using biomass as fuel through electronic devices with great potential to generate electricity.“
  • Cargando...
    Miniatura
    PublicaciónAcceso abierto
    Microbial Biosensors for Wastewater Monitoring: Mini-Review
    (MDPI, 2022-10-04) Rojas-Villacorta, Walter; Rojas-Flores, Segundo; De La Cruz-Noriega, Magaly; Chinchay Espino, Héctor; Diaz, Felix; Gallozzo Cardenas, Moises
    “Research on the use of microbial biosensors for monitoring wastewater contaminants is a topic that covers few publications compared to their applicability in other fields, such as biomedical research. For this reason, a systematic analysis of the topic was carried out, for which research-type articles were reviewed during the period 2012 to September 2022. For this, different search platforms were used, including PubMed, ScienceDirect, Springer Link, and Scopus, and through the use of search equations a relevant bibliography was located. After that, the research articles were selected based on exclusion criteria. As a result, it was found that, of the 126 articles, only 16 articles were strictly related to the topic, since there was a duplication of articles among the different databases. It was possible to demonstrate the usefulness of microorganisms as components of biosensors to monitor BOD, heavy metals, and inorganic contaminants in wastewater that also had a high sensitivity. Additionally, recombinant DNA techniques were shown to improve the performance of this type of biosensor and can finally be coupled to other emerging technologies, such as microbial fuel cells (MFCs). In conclusion, it was established that microbial biosensors have high acceptability and monitoring characteristics that make them a useful tool to detect low concentrations of pollutants in wastewater that can also provide results in real-time, thus generating forms of ecological safety and social responsibility in companies where wastewater is generated.“
  • Cargando...
    Miniatura
    PublicaciónAcceso abierto
    Microbial Biosensors for Wastewater Monitoring: Mini-Review
    (MDPI, 2022-10-04) Rojas-Villacorta, Walter; Rojas-Flores, Segundo; De La Cruz-Noriega, Magaly; Chinchay Espino, Héctor; Diaz, Felix; Gallozzo Cardenas, Moises
    “Research on the use of microbial biosensors for monitoring wastewater contaminants is a topic that covers few publications compared to their applicability in other fields, such as biomedical research. For this reason, a systematic analysis of the topic was carried out, for which research-type articles were reviewed during the period 2012 to September 2022. For this, different search platforms were used, including PubMed, ScienceDirect, Springer Link, and Scopus, and through the use of search equations a relevant bibliography was located. After that, the research articles were selected based on exclusion criteria. As a result, it was found that, of the 126 articles, only 16 articles were strictly related to the topic, since there was a duplication of articles among the different databases. It was possible to demonstrate the usefulness of microorganisms as components of biosensors to monitor BOD, heavy metals, and inorganic contaminants in wastewater that also had a high sensitivity. Additionally, recombinant DNA techniques were shown to improve the performance of this type of biosensor and can finally be coupled to other emerging technologies, such as microbial fuel cells (MFCs). In conclusion, it was established that microbial biosensors have high acceptability and monitoring characteristics that make them a useful tool to detect low concentrations of pollutants in wastewater that can also provide results in real-time, thus generating forms of ecological safety and social responsibility in companies where wastewater is generated.“
  • Cargando...
    Miniatura
    PublicaciónAcceso abierto
    Preliminary Study of Bioelectricity Generation Using Lettuce Waste as Substrate by Microbial Fuel Cells
    (MDPI, 2023-06-30) Rojas-Villacorta, Walter; Rojas-Flores, Segundo; Benites, Santiago M.; Nazario-Naved, Renny; Romero, Cecilia V.; Gallozzo-Cardenas, Moisés; Delfín-Narciso, Daniel; Díaz, Félix; Murga-Torres, Emzon
    “Agricultural waste negatively impacts the environment and generates economic difficulties for agro-industrial companies and farmers. As a result, it is necessary for an eco-friendly and sustainable alternative to managing this type of waste. Therefore, the research aimed to investigate lettuce waste as an alternative substrate to generate bioelectricity in single-chamber microbial fuel cells (scMFCs). It was possible to report voltage and electric current peaks of 0.959 ± 0.026 V and 5.697 ± 0.065 mA on the fourteenth day, values that were attained with an optimum pH of 7.867 ± 0.147 and with an electrical conductivity of 118.964 ± 8.888 mS/cm. Moreover, as time passed the values began to decline slowly. The calculated value of maximum power density was 378.145 ± 5.417 mW/cm2 whose current density was 5.965 A/cm2 , while the internal resistance reported using Ohm’s Law was 87.594 ± 6.226 Ω. Finally, it was possible to identify the Stenotrophomonas maltophilia bacterium (99.59%) on a molecular scale, as one of the microorganisms present in the anodic biofilm. The three microbial fuel cells were connected in series and demonstrated that they were capable of lighting an LED bulb, with a voltage of 2.18 V.“
  • Cargando...
    Miniatura
    PublicaciónAcceso abierto
    Preliminary Study of Bioelectricity Generation Using Lettuce Waste as Substrate by Microbial Fuel Cells
    (MDPI, 2023-06-30) Rojas-Villacorta, Walter; Rojas-Flores, Segundo; Benites, Santiago M.; Nazario-Naveda, Renny; Romero, Cecilia V.; Gallozzo-Cardenas, Moisés; Delfín-Narciso, Daniel; Díaz, Félix; Murga-Torres, Emzon
    Agricultural waste negatively impacts the environment and generates economic difficulties for agro-industrial companies and farmers. As a result, it is necessary for an eco-friendly and sustainable alternative to managing this type of waste. Therefore, the research aimed to investigate lettuce waste as an alternative substrate to generate bioelectricity in single-chamber microbial fuel cells (scMFCs). It was possible to report voltage and electric current peaks of 0.959 ± 0.026 V and 5.697 ± 0.065 mA on the fourteenth day, values that were attained with an optimum pH of 7.867 ± 0.147 and with an electrical conductivity of 118.964 ± 8.888 mS/cm. Moreover, as time passed the values began to decline slowly. The calculated value of maximum power density was 378.145 ± 5.417 mW/cm2 whose current density was 5.965 A/cm2 , while the internal resistance reported using Ohm’s Law was 87.594 ± 6.226 Ω. Finally, it was possible to identify the Stenotrophomonas maltophilia bacterium (99.59%) on a molecular scale, as one of the microorganisms present in the anodic biofilm. The three microbial fuel cells were connected in series and demonstrated that they were capable of lighting an LED bulb, with a voltage of 2.18 V.
  • Cargando...
    Miniatura
    PublicaciónAcceso abierto
    Use of Kiwi Waste as Fuel in MFC and Its Potential for Use as Renewable Energy
    (MDPI, 2023-04-12) Rojas-Flores, Segundo; De La Cruz-Noriega, Magaly; Cabanillas-Chirinos, Luis; Benites, Santiago M.; Nazario-Naveda, Renny; Delfín-Narciso, Daniel; Gallozzo-Cardemas, Moisés; Murga-Torres, Emzon; Rojas-Villacorta, Walter; Díaz, Felix
    This research aimed to use kiwi waste as fuel to generate bioelectricity through microbial fuel cells. It was possible to generate an electrical current and voltage peaks of 3.807 ± 0.102 mA and 0.993 ± 0.061 V on day 11, showing an electrical conductivity of 189.82 ± 3.029 mS/cm and an optimum operating pH of 5.966 ± 0.121. The internal resistance of the cells was calculated using Ohm’s Law, resulting in a value of 14.957 ± 0.394 Ω, while the maximum power density was 212.68 ± 26.84 mW/m2 at a current density of 4.506 A/cm2. Through the analysis of the FTIR spectra carried out on the substrate, a decrease in the characteristic organic peaks was observed due to their decomposition during the electricity-generation process. In addition, it was possible to molecularly identify the bacteria Comamonas testosteroni, Sphingobacterium sp., and Stenotropho-monas maltophila adhered to the anodized biofilm. Finally, the capacity of this residue to generate bioelectricity was demonstrated by lighting an LED bulb with a voltage of 2.85 V.
  • Cargando...
    Miniatura
    PublicaciónAcceso abierto
    Use of Kiwi Waste as Fuel in MFC and Its Potential for Use as Renewable Energy
    (MDPI, 2023-04-08) Rojas-Flores, Segundo; De La Cruz-Noriega, Magaly; Cabanillas-Chirinos, Luis; Benites, Santiago M.; Nazario-Naveda, Renny; Delfín-Narciso, Daniel; Gallozzo-Cardemas, Moisés; Díaz, Felix; Murga-Torres, Emzon; Rojas-Villacorta, Walter
    This research aimed to use kiwi waste as fuel to generate bioelectricity through microbial fuel cells. It was possible to generate an electrical current and voltage peaks of 3.807 ± 0.102 mA and 0.993 ± 0.061 V on day 11, showing an electrical conductivity of 189.82 ± 3.029 mS/cm and an optimum operating pH of 5.966 ± 0.121. The internal resistance of the cells was calculated using Ohm’s Law, resulting in a value of 14.957 ± 0.394 Ω, while the maximum power density was 212.68 ± 26.84 mW/m2 at a current density of 4.506 A/cm2. Through the analysis of the FTIR spectra carried out on the substrate, a decrease in the characteristic organic peaks was observed due to their decomposition during the electricity-generation process. In addition, it was possible to molecularly identify the bacteria Comamonas testosteroni, Sphingobacterium sp., and Stenotropho-monas maltophila adhered to the anodized biofilm. Finally, the capacity of this residue to generate bioelectricity was demonstrated by lighting an LED bulb with a voltage of 2.85 V.
  • Cargando...
    Miniatura
    PublicaciónAcceso abierto
    “Use of Tangerine Waste as Fuel for the Generation of Electric Current“
    (MDPI, 2023-02-15) Ortiz-Saavedra, Brando; Cabanillas-Chirinos, Luis; Nazario-Naveda, Renny; Gallozzo-Cardenas, Moisés; Diaz, Félix; Delfin-Narciso, Daniel; Rojas-Villacorta, Walter
    “Fruit waste has increased exponentially worldwide, within which tangerine is one of those that generates a greater amount of organic waste, which is currently not fully used. On the other hand, microbial fuel cells (MFCs) are presented as an opportunity to take advantage of organic waste to generate electricity, which is why the main objective of this research is to generate bioelectricity using tangerine waste as a substrate in microbial fuel cells using zinc and copper electrodes. It was possible to generate current and voltage peaks of 1.43973 ± 0.05568 mA and 1.191 ± 0.035 V on days eighteen and seventeen, respectively, operating with an optimum pH of 4.78 ± 0.46 and with electrical conductivity of the substrate of 140.07 ± 3.51 mS/cm, while the Brix degrees gradually decreased until the last day. The internal resistance determined was 65.378 ± 1.967 Ω, while the maximum power density was 475.32 ± 24.56 mW/cm2 at a current density of 5.539 A/cm2 with a peak voltage of 1024.12 ± 25.16 mV. The bacterium (Serratia fonticola) and yeasts (Rhodotorula mucilaginosa) were identified in the substrate with an identity of 99.57 and 99.50%, respectively. Finally, the cells were connected in series, managing to generate 3.15 V, which allowed the turning on of a red LED light. “
  • Cargando...
    Miniatura
    PublicaciónAcceso abierto
    “Use of Tangerine Waste as Fuel for the Generation of Electric Current“
    (MDPI, 2023-02-15) Rojas-Flores, Segundo; Cabanillas-Chirinos, Luis; Nazario-Naveda, Renny; Gallozzo-Cardenas, Moisés; Diaz, Félix; Delfin-Narciso, Daniel; Rojas-Villacorta, Walter
    “: Fruit waste has increased exponentially worldwide, within which tangerine is one of those that generates a greater amount of organic waste, which is currently not fully used. On the other hand, microbial fuel cells (MFCs) are presented as an opportunity to take advantage of organic waste to generate electricity, which is why the main objective of this research is to generate bioelectricity using tangerine waste as a substrate in microbial fuel cells using zinc and copper electrodes. It was possible to generate current and voltage peaks of 1.43973 ± 0.05568 mA and 1.191 ± 0.035 V on days eighteen and seventeen, respectively, operating with an optimum pH of 4.78 ± 0.46 and with electrical conductivity of the substrate of 140.07 ± 3.51 mS/cm, while the Brix degrees gradually decreased until the last day. The internal resistance determined was 65.378 ± 1.967 Ω, while the maximum power density was 475.32 ± 24.56 mW/cm2 at a current density of 5.539 A/cm2 with a peak voltage of 1024.12 ± 25.16 mV. The bacterium (Serratia fonticola) and yeasts (Rhodotorula mucilaginosa) were identified in the substrate with an identity of 99.57 and 99.50%, respectively. Finally, the cells were connected in series, managing to generate 3.15 V, which allowed the turning on of a red LED light.“
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